2017

2017/7/4-7に、Mahidol University, Bangkok, Thailandにて、日本学術振興会研究拠点形成事業「2nd Technology Seminar on the MinION sequencing」を開催致しました。

Summary

2nd Technology Seminar on the MinION sequencing

Title: Incorporation of DNA sequencing in various health problems in Thailand

Background: Sequencing has come a long way. Being the definitive tools for genetics, sequencing is important to detect mutations, identify microorganism, even uncover the secrets of our past. The sequencing tools have transformed from huge equipment, to benchtop machines, and finally has been miniaturized into a size of a chocolate bar. Using MinION, we have finally achieved the freedom to sequence right in the field. MinION has successfully reduced sequencing technology to a portable, easy to perform, and reasonably cost-effective machine that only needs 1-2 hours of hands-on procedure. Utilizing this technology, we would embark on a journey to detect various health problems in Thailand.

Topics:
1. Identification of insecticide resistance. Aedes aegypti is the vector of dengue virus, one of the most important arboviruses. The control of this virus many times is hampered by the appearance of insecticide resistance mosquitoes. The resistance to pyrethroid is due to mutations in the voltage-gated sodium channel gene. Using MinION, we would try to detect the mutations in exons 19-27 of the gene using samples from Thailand.
2. Identification of artemisinin resistance.Malaria is among the top 10 causes of death in low-income countries. Plasmodium falciparum as the cause of malaria is elusive. Vaccine is still not available and the only medicine effective to kill it has been found to be less effective. The cause for this resistance is various mutations in the propeller domains of K13 gene. MinION is very cost-effective to detect mutations in this gene and help with the diagnosis of artemisinin-resistance malaria.
3. Identification of microorganism.Microorganism is an inseparable aspect of our life. From commensal to parasitic microorganisms, we are in constant exposure to them. Identifying them is important in the management of sepsis or to prevent food poisoning. In this area, we could also incorporate MinION. We would sequence the bacterial 16s rDNA and come out with the list of bacteria found in yoghurt.

Schedule

July 4 th :Hands-on-Training@Faculty of Tropical Medicine, Mahidol University
9:00 – 9:10 Opening ceremony by Yutaka Suzuki (The University of Tokyo)
9:10 – 9:20 Welcome message by Narumon Komalamisra (Mahidol University)
9:20 – 10:00 Introduction to MinION by Mari Miyamoto (Oxford Nanopore Technologies)
10:00 – 12:00 DNA extraction from mosquitoes, malaria-infected blood, and yoghurt
12:00 – 13:30 Lunch
13:30 – 17:00 DNA amplification
18:00 - Welcome Party

July 4th Welcome Party @Mahidol University

July 5th :Hands-on-Training@Ramathibodhi Hospital Faculty of Medicine, Mahidol University
10:00 – 12:00 MinION library preparation
12:00 – 13:30 Lunch
13:30 – 14:30 Sequencing
14:30 – 17:00 Introduction to Bioinformatics by Wojlech Makalowski (University of Münster)

July 6th :Symposium@Faculty of Tropical Medicine, Mahidol University
9:00-9:30 Opening remarks
Yutaka Suzuki(The University of Tokyo, Japan)
9:30-10:45 Plenary I: Nanopore sequencing and its analysis
Mari Miyamoto(Oxford Nanopore Technologies, Japan)
Wojtek Makalowski( University of Muenster, Germany)
Martin Frith(The University of Tokyo, Japan)
10:45-11:00 Break
11:00-12:00 Plenary II: One Health approach to tackle the health problems
Wayan Artama (Gajah Mada University/INDOHUN, Indonesia)
Saengduen Moonsom (Mahidol University/THOHUN, Thailand)
12:00-13:00 Luncheon seminar by Fujimori Kogyo CO., LTD.
13:00-14:00 Plenary III: Molecular application in entomology
Josef S. B. Tuda(Sam Ratulangi University, Indonesia)
Raweewan Srisawat(Mahidol University, Thailand)
Yuki Eshita(Hokkaido University, Japan)
14:00-15:00 Plenary Ⅳ: Updates on molecular biology
Masato Suzuki(The National Institute of Infectious Diseases, Japan)
Takuya Maeda (Saitama Medical University)
Satomi Mitsuhashi(Yokohama City University, Japan)
15:00-15:15 Coffee break
15:15-16:30 Plenary V : New findings in Science
Nguyen Tuan Nghia (National Institute of Hygiene and Epidemiology, Vietnam)
Nuankanya Sathirapongsasuti(Mahidol University, Thailand)
Sanaz Firouzi(The University of Tokyo, Japan)
TBD from King Abdullah University of Science & Technology, Saudi Arabia
Cheong Jieun(Dong-a University, Korea)
16:30-16:40 Closing remarks
Narumon Komuramisra (Mahidol Universty, Thailand)

July 7th :Hands-on-Traninig@Ramathibodhi Hospital Faculty of Medicine, Mahidol University
10:00 – 12:00 Data analysis
12:00 – 13:30 Lunch
13:30 – 16:00 Data analysis
16:00 – 17:00 Wrap-up meeting