Hands on Training program

Venue:Auditorium of Sam Ratulangi University, Manado, Indonesia

Date: July 4-7, 2018

Day I: Opening ceremony, DNA and RNA extraction
Day II: MinION library preparation and sequencing
Day III: Data analysis

Topics:
1. Drug resistance screening in malaria parasite.
Malaria is among the top 10 causes of death in low-income countries. Plasmodium falciparum as the cause of malaria is elusive. Vaccine is still not available and the only medicine effective to kill it has been found to be less effective. The cause for this resistance is various mutations in the propeller domains of K13 gene, among other genes. MinION is very cost-effective to detect mutations in this gene and help with the diagnosis of artemisinin-resistance malaria and other drug-resistance.
2. Bacterial identification from clinical samples.
Microorganism is an inseparable aspect of our life. From commensal to parasitic microorganisms, we are in constant exposure to them. Identifying them is important in the management of sepsis or to prevent food poisoning. In this area, we could also incorporate MinION. We would sequence the bacterial 16s rDNA and come out with the list of bacteria found in saliva or blood.
3. Cancer mutation sequencing.
Various types of mutations have been detected in lung cancer genome. Using MinION, we will try to detect the deletion of EGFR, SNV of KRAS and fusion transcripts of ALK and RET in lung cancer cell lines. Further, by phasing the T790M and L858R mutations, we can find out the resistance of lung adenocarcinoma to gefitinib and erlotinib and can plan to change therapy to double-mutant sensitive drugs AZD9291.